Practicals Syllabus, Notes & PYQ for GGSIPU Doctor of Medicine Overview

Doctor of Medicine
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Practicals
1Specimen collection, handling and storage of samples.
2Method standardization, evaluation and calibration.
3Methods for quality control and assessment.
4Preparation and storage of reagents.
5Fractionation & identification of amino acids, sugars, proteins and lipoproteins by Thin Layer chromatography.
6Fractionation & identification of amino acids, sugars, proteins and lipoproteins by Gel electrophoresis — agarose, starch, polyacrylamide.
7Fractionation & identification of amino acids, sugars, proteins and lipoproteins by Paper electrophoresis.
8Urine analysis.
9Enzyme kinetics; isoenzyme separation by polyacrylamide gel electrophoresis. Study of AST, ALT, Alkaline phosphatase, Acid phosphatase, Amylase, Creatine kinase.
10Isolation and characterization of biochemical substances from tissues: Glycogen from liver.
11Isolation and characterization of biochemical substances from tissues: Cholesterol from brain.
12Isolation and characterization of biochemical substances from tissues: Casein from milk.
13Isolation and characterization of biochemical substances from tissues: DNA isolation.
14Isolation and characterization of biochemical substances from tissues: Haemoglobin from blood.
15Isolation and characterization of biochemical substances from tissues: RNA isolation.
16Estimation of serum total cholesterol.
17Estimation of serum HDL cholesterol.
18Estimation of serum VLDL cholesterol.
19Estimation of serum triglycerides.
20Urine stone analysis.
21Estimation of iron and TIBC.
22Estimation of glycosylated Hb.
23Orientation to auto analysers and basic techniques of PCR, ELISA, RIA, HPLC and blotting.